Generator
Part:BBa_I716340:Design
Designed by: nhu nguyen Group: iGEM07_Berkeley_UC (2007-08-14)
rbs-thpD-TT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1059 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal NgoMIV site found at 666 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 483
Illegal SapI site found at 722
Design Notes
Digest I716338 (EcoRI/BamHI, 2057bp, 923bp, S) Sub into Sub into pBca9145-Bca1092 (EcoRI/BglII 2198bp, 9bp, L) Product is I716340
Source
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.